Former graduate student
- Ph.D. Oceanography. University of Washington, Seattle, WA, 2019.
- M.Sc. Oceanography. University of Washington, Seattle, WA, 2015.
- B.Sc. Environmental Science (Hon.), Biology minor. Mount Allison University, Sackville, NB, 2011.
- , Amin, S.A., Armbrust, E.V. 2016. Ubiquitous marine bacterium inhibits diatom cell division. The ISME Journal. 1-12.
- Amin, S.A., Hmelo, L.R., , Durham, B.P., Carlson, L.T., Heal, K.R., Morales, R.L., Berthiaume, C.T., Parker, M.S., Djunaedi, B., Ingalls, A.E., Parsek, M.R., Moran, M.A., and Armbrust, E.V. 2015. Interaction and signalling between a cosmopolitan phytoplankton and associated bacteria. Nature. 522: 98-101.
- , Irwin, A.J., and Finkel, Z.V. 2012. Macroevolutionary trends in silicoflagellate skeletal morphology: the costs and benefits of silicification. Paleobiology. 38(3): 391-402.
Constraint-based metabolic modeling of interaction between Thalassiosira pseudonana and Ruegeria pomeroyi:
For my Ph.D., I created genome-scale metabolic models of the diatom *Thalassiosira pseudonana* CCMP 1335 and the bacterium *Ruegeria pomeroyi* DSS-3 to investigate the dynamics of interaction between these two model marine organisms under different nutrient conditions.
Constraint-based metabolic models can be used to study the flux of metabolites and nutrients in marine microbes, connecting molecular information to the environment. These models have been used to integrate a wealth of different information types and to predict the metabolic behaviour of many different organisms.
In culture, growth of the diatom *Thalassiosira pseudonana* can be supported by vitamin B12-producing bacteria such as *Ruegeria pomeroyi*. In exchange *R. pomeroyi* receives organic carbon and sulfur from *T. pseudonana*. Vitamin B12 is the only organic compound required by most diatoms and it has been demonstrated that *R. pomeroyi* consumes 2,3-dihydroxypropane sulfonate (DHPS) and N-acetyltaurine produced by *T. pseudonana*, although there may be other molecules exchanged between these two organisms.
I used these models to study the dynamics of this interaction and investigate the possible exchange of other molecules.
Effect of Croceibacter atlanticus on cell division in Thalassiosira pseudonana:
For my Master's project I studied interactions between the flavobacterium *Croceibacter atlanticus* and diatoms. *C. atlanticus* was isolated from the pennate chain-forming diatom *Pseudo-nitzschia multiseries*, and was found to inhibit growth in many different diatoms. When *C. atlanticus* was grown with the centric diatom *Thalassiosira pseudonana*, we observed large polyploid cells with multiple plastids. These features indicate that *C. atlanticus* may inhibit cytokinesis in diatoms.