Shiri Graff van Creveld

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Postdoctoral Research Associate

shirig at uw.edu

A molecular biologist by training, in my PhD I developed tools, and studied responses to environmental stresses in a well-established model diatom in the lab. Now I am studying the molecular mechanisms underlying diatoms acclimation and stress response from model species to ecosystems, coupling laboratory studies with metatranscriptomes and novel isolates from the Pacific Ocean.

Education

  • PhD, Life Science, Weizmann Institute of Science, Israel, 2019
  • MSc, Life Science, Weizmann Institute of Science, Israel, 2013
  • BSc, Molecular Biochemistry, The Technion – Israel Institute of Technology, Israel, 2010

Publications

  • Graff van Creveld S, Coesel S, Schatz M, Armbrust EV (2024) New eukaryotic phytoplankton isolates from the oligotrophic tropical Pacific Ocean. J. Phycol. https://doi.org/10.1111/jpy.13447
  • Graff van Creveld S, Coesel S, Blaskowski S, Groussman R, Schatz M, Armbrust EV (2023) Divergent functions of two clades of flavodoxin in diatoms mitigate oxidative stress and iron limitation. eLife https://doi.org/10.7554/eLife.84392
  • Bartolek Z, Graff van Creveld S, Coesel S, Cain K, Schatz M, Morales R, Armbrust EV (2022) Flavobacterial exudates disrupt cell cycle progression and metabolism of the diatom Thalassiosira pseudonana. ISME J. https://doi.org/10.1038/s41396-022-01313-9
  • Graff van Creveld S, Mizrachi A, Vardi A (2022) “An ocean of signals: intracellular and extracellular signaling in diatom” in: The Molecular Life of Diatoms, Eds Falciatore A and Mock T, Springer Nature (London, England). https://doi.org/10.1007/978-3-030-92499-7_22
  • Graff van Creveld S, Ben-dor S, Mizrachi A, Alcolombri U, Hopes A, Mock T, Rosenwasser S, Vardi A (2021) Biochemical Characterization of a Novel Redox-Regulated Metacaspase in a Marine Diatom. Front. Microbiol. https://doi.org/10.3389/fmicb.2021.688199
  • Mizrachi A, Graff van Creveld S, Shapiro OH, Rosenwasser S, Vardi A (2019) Light-dependent single-cell heterogeneity in the chloroplast redox state regulates cell fate in a marine diatom. eLife 8:e47732
  • Stock F, Syrpas M, Graff van Creveld S, Backx S, Blommaert L, Dow L, Stock W, Ruysbergh E, Lepetit B, Bailleul B, et al (2019) N-acyl homoserine lactone derived tetramic acids impair photosynthesis in Phaeodactylum tricornutum. ACS Chem Biol acschembio.8b01101
  • Volpert A, Graff van Creveld S, Rosenwasser S, Vardi A (2018) Diurnal fluctuations in chloroplast GSH redox state regulate susceptibility to oxidative stress and cell fate in a bloom-forming diatom. J Phycol. 54: 329–341. https://doi.org/10.1111/jpy.12638
  • Rosenwasser S, Ziv C, Graff van Creveld S, Vardi A (2016) Virocell Metabolism: Metabolic Innovations During Host–Virus Interactions in the Ocean. Trends Microbiol 24: 821–832. https://doi.org/10.1016/j.tim.2016.06.006
  • Graff van Creveld S, Rosenwasser S, Levin Y, Vardi A (2016) Chronic iron limitation confers transient resistance to oxidative stress in marine diatoms. Plant Physiol. 172: 968–979. https://doi.org/10.1104/pp.16.00840
  • Graff van Creveld S, Rosenwasser S, Schatz D, Koren I, Vardi A (2015) Early perturbation in mitochondria redox homeostasis in response to environmental stress predicts cell fate in diatoms. ISME J. 9: 385–395. https://doi.org/10.1038/ismej.2014.136
  • Rosenwasser S, Graff van Creveld S, Schatz D, Malitsky S, Tzfadia O, Aharoni A, Levin Y, Gabashvili A, Feldmesser E, Vardi A (2014) Mapping the diatom redox-sensitive proteome provides insight into response to nitrogen stress in the marine environment. Proc Natl Acad Sci. 111: 2740–5. https://doi.org/10.1073/pnas.1319773111

Cruise participation

  • 22 Aug - 26 Aug 2024, Honolulu, HI. R/V Kilo Moana KM24-14, Chief Scientist: Angelicque E. White, University of Hawaii. NSF-UNOLS Early Career Chief Scientist Training Cruise
  • 22 Jan - 18 Feb 2023, San Diego, CA - Honolulu, HI. R/V Thompson TN412, Chief Scientist: E. Virginia Armbrust, University of Washington
  • 11 Nov - 15 Dec 2021, Seattle, WA - San Diego, CA - Honolulu, HI. R/V Thompson TN396 TN397, Chief Scientist: E. Virginia Armbrust, University of Washington
  • 21 Apr - 4 May 2012, Woods Hole, MA - Bermuda, R/V Knorr KN203-1, Chief Scientist: Ben Van Mooy, Woods Hole Oceanographic Institute

Scientific resources

Phytoplankton strains Phytoplankton isolated from the Pacific Ocean deposited in public culture collections: Thirty-six phytoplankton strains are available at the National Center for Marine Algae and Microbiota, USA NCMA. For example, Pseudo-nitzschia sp, CCMP3171, Planktoniella sol, CCMP3726.

Plasmids Plasmids deposited in Addgene, for gene editing in Phaeodactylum triconutum